PHYSICAL AND ENZYMATIC PRE-TREATMENTS OF MICROALGACEA BIOMASS

Name: Tamiris Vescovi Casotto
Type: MSc dissertation
Publication date: 24/08/2022
Advisor:

Namesort descending Role
Sérvio Túlio Alves Cassini Advisor *

Examining board:

Namesort descending Role
Jairo Pinto de Oliveira External Examiner *
Larissa Bernardino Moro Co advisor *
Regina de Pinho Keller Internal Examiner *
Sérvio Túlio Alves Cassini Advisor *

Summary: A fundamental step for the use of energy and biorenewable co-products of high economic value from
microalgae is the cellular cell. The objective of this work was to evaluate physical and enzymatic pretreatment without damage to the cell wall of microalgae. Manual milling, ultrasound, microwave and
enzymatic hydrolysis were the treatments tested in a simple and combined way. Were used unused in
the control process and positive processing/ml, concentrates in processing by the solid-state
fermentation (SSF) process used in the pre-transplanted handling process, concentrates in a positive
processing/ml process, processed by the fermentation process, in solid state (FS) fungus C1 genus and
unidentified species, and a crude extract with an activity of 1.74 U/ml, produced by the fungus
Aspergillus niger (MAR). enzymatic hydrolysis of the biomass of Chlorella vulgaris using a mixture with
the two enzymatic types and at least one physical method of cell stimulation A. The highest efficiencies
achieved ranged from 32.94 to 40.1%, with both ultrasound, as of enzymatic hydrolysis and took
periods of 1 or 2 hours to achieve these results. All real features tested, which reached 18.99%
efficiency. The enzymatic results and the potential use of ultrasound, promissory treatment, allowed
the use of physical and biological pre-treatments, in order to facilitate the use of cellular products from
biomass microalgae.

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