Name: Paulo Wagnner Pereira Antunes
Type: PhD thesis
Publication date: 22/04/2013

Namesort descending Role
Sérvio Túlio Alves Cassini Advisor *

Examining board:

Namesort descending Role
Laura Marina Pinotti Internal Examiner *
Paulo Waldir Tardioli External Examiner *
Regina de Pinho Keller Internal Examiner *
Sérvio Túlio Alves Cassini Advisor *

Summary: The monitoring program performed with surface water samples of the
metropolitan region of Vitoria, ES, showed the presence of one variant of
microcystin in 57% of samples. With 20% of these samples the concentration of
microcystin was greater than 1.0 μg / L, the maximum concentration allowed by
Brazilian legislation for public water supply. Few laboratories in the state of
Espírito Santo have appropriate infrastructure for the analysis of microcystins
and furthermore, the current quantitative methods are costly and time
consuming. With the main objective to develop a system for microcystin
detection, in this study a quantitative method was validated by high
performance liquid chromatography (HPLC) and established a qualitative
system (P / A) for analysis of microcystins. The validated method free of
acetonitrile showed linearity and selectivity to separate and quantify different
microcystin variants (-RR,-YR,-LR and-LA). Recovery levels between 98.2 and
106.1% have demonstrated the precision and detection limits (from 0.17 to 0.25
g / L) and quantification (between 0.55 and 0.82 g / L) met the national and
international standard limits for drinking water. The qualitative system (P / A)
was proven to be easy to use, low cost and high sensitivity, allowing direct
visual determination of the presence of microcystin in concentrations above
0.80 mg / L, without the need for any processing method , cleaning or
concentration of the sample. Compared with traditional microcystin detection
methods such as ELISA and HPLC it was shown the system reliability rates of
82.4% and 88.2%.
Keywords: Microcystin detection methods, water quality and monitoring

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