Name: Sara Angelino Martins
Type: MSc dissertation
Publication date: 10/07/2013
Advisor:
| Name | Role |
|---|---|
| Regina de Pinho Keller | Advisor * |
Examining board:
| Name | Role |
|---|---|
| Regina de Pinho Keller | Advisor * |
| Tulio Machado Fumian | External Examiner * |
| Sérvio Túlio Alves Cassini | Internal Examiner * |
Summary: The region of the Ilha das Caieiras, in Vitória (ES) is located within an estuarine
region in Vitoria Bay and is close to an extensive mangrove area. The resident
population ratio remains highly dependent relationship on natural resources
offered (oysters, clams) therefore used as a source of subsistence and
recreation. The mangrove of Ilha das Caieiras receives sewage from adjacent
areas, contaminating water and shellfish therein. In addition, microorganisms
that are cast together with the sewage in the coastal environment can be
accumulated in the sediment. This objective of this work was to investigate
enteric viruses and bacterial indicators of fecal pollution in the sediment and
water at three points of this estuary (P1, P2 and P3) mangrove area over 15
months of monitoring. We evaluated a total of 45 samples for the presence of
thermotolerant coliform (TC), enterococci and enteric viruses (adenovirus -
HAdV; rotavirus - RVA, and norovirus - NoV GII, using a nested PCR. We also
evaluated the physico-chemical parameters of the water collection point next to
the sediment. Microbiological analyzes of the sediment were respectively
geometric mean for enterococci: 1.5 x103, 1.5 x103 and 1.1 x103 UFC/100g,
thermotolerantes coliforms: 4.9 x103, 2.6 x102 and 5.1 x102. In water samples
the results for enterococci were: 7.6 x101, 5.0 x101 and 1.1 x102 and
themortolerants coliforms: 2.2 x102, 1.3 x102, and 3.0 x102. Analyses of enteric
viruses in water samples showed frequencies of 20% for RV, 13% for AdV and
8.8% for NoV GII and sediment samples only NoV GII (4.5%) was found. The
presence of the viral and bacteriological samples indicates that this region is
under anthropogenic impact, and that the sediment from the mangrove of Ilha
das Caieiras can act as a reservoir of pollutants that are released to the water
column can cause gastrointestinal diseases the people who use this area as a
source of livelihood and recreation.
Keywords: Mangrove, Sediment, Water, Enteric Viruses, Coliforms
themotolerant, Nested-PCR.
